原创《烟秆生物质炭对烟草根际土壤养分细菌群落影响》：本文关于烟秆生物质炭论文范文,可以做为相关论文参考文献,与写作提纲思路参考。
摘 要：通過田间小区试验研究了不同生物质炭用量对植烟土壤养分、微生物多样性和菌群丰度的影响.结果表明,梯度施入烟秆生物质炭提升了酸性土壤pH,促进了土壤有机质及全氮的积累,随着生物质炭用量的加大,土壤pH、有机质含量均有逐步升高的趋势,土壤速效钾释放速率也得到提高.施用烟秆生物质炭后,烟草根际土壤的微生物种类（OTU数）提高了26.4%.优势菌种中,变形菌门所占比例最大,达到47.19%～54.32%.生物质炭施用下,部分有利植物生长的促生菌呈增长趋势.将烟秆生物质炭用于烟田土壤改良,既可提升土壤肥力,又可缓解烟秆不合理利用导致的环境污染问题,但烟秆生物质炭推广应用还有一定的局限性.
关键词：烟秆生物质炭；根际土壤；土壤养分；促生菌；高通量测序
中图分类号：S572.062文章编号：1007-5119（2017）01-0042-06DOI：10.13496/j.issn.1007-5119.2017.01.007
Abstract：The field experiments were conducted to study the impacts of tobacco stalk-derived biochar at different levels on soil nutrients, microbial diversity and abundance of flora. The results showed that gradient application of tobacco biochar increased pH of acidic soil,promoted soil organic matter and total N accumulation gradually, and improved the releasing of available K. The results also indicated that the amount of rhizosphere soil microbes increased after the application of biochar, and the greatest increment （3.25 times） was achieved by the group at the level of 80t/hm2. The operational taxonomic unit （OTU） increased by 26.4%. The OTU analysis on bacteria at 97% similarity demonstrated that there was no difference at the top 10 dominant phyla between the groups at different levels. The highest distribution was observed in Proteobacteria that accounted for 47.19% to 54.32% of the total bacteria. This association was also applied to the increase of plant growth-promoting microorganisms （PGPMs）. The applications of tobacco biochar can not only improve the soil fertility, but also ease the environmental pollution problems caused by unreasonable use of tobacco stems. However some limitations also existed in the popularization and application of tobacco stalk-derived biochar in tobacco agriculture.
Keywords：tobacco stalk-derived biochar； rhizosphere soil； soil nutrients； PGPMs； high throughput sequencing
我国烤烟年产量约273万t[1],若按烟叶和烟秆1：1的比例粗略计算[2],每年约产生273万t烟秆.烟秆随意丢弃或焚烧,不但会传播烟草病害,同时会对生态环境造成不利影响,因此烟秆等废弃物的无害化处理成为烟草农业面临的重要难题[3].生物质炭是在限氧条件下将生物质材料热解碳化后产生的高度芳香化富含碳的固态物质[4].将生物质炭作为土壤改良剂应用于农业生产,既可改善土壤理化性质、提升土壤肥力、提高作物生产力[5-6],又可提升土壤碳库、减少温室气体排放[7].近年来,利用木醋液[8]、花生壳炭[9-11]、稻壳炭[12-13]、小麦秸秆炭[14]和玉米秸秆炭[15]等改良烟田土壤、改善烤烟品质的研究已多有报道,但将烟秆炭化并用于烟田土壤改良的报道还较少,本文以调控烟田连作障碍为切入点,将烟秆制备成生物质炭应用于烟田土壤改良,既提升了烟田土壤肥力,又缓解了烟秆不合理利用导致的环境污染问题,对促进烟草农业持续健康发展具有重要作用.
1 材料和方法
1.1 试验地概况
田间试验位于福建省福州市晋安区宦溪镇福建省烟草农业科研基地（119°36′86″E,26°17′33″N）.烟田土壤为红壤性水稻土,耕作模式为烟稻年内轮作年际连作.试验前土壤养分状况：pH 5.45,有机质22.60 g/kg,碱解氮103.00 mg/kg,有效磷31.31 mg/kg,速效钾109.95 mg/kg[16].
烟秆生物质炭论文参考资料：
结论：烟秆生物质炭对烟草根际土壤养分细菌群落影响为关于本文可作为烟秆生物质炭方面的大学硕士与本科毕业论文生物质炭论文开题报告范文和职称论文论文写作参考文献下载。
