原创《甘蔗ScHTD2基因克隆生物信息学分析》：该文是关于基因克隆论文范文，为你的论文写作提供相关论文资料参考。
摘 要 D14是一类能够有效抑制水稻分蘖的水解酯酶,可能是独脚金内酯信号传导途径中的重要组分.为研究该基因在甘蔗分蘖性状中的功能,利用RT-PCR和RACE技术从甘蔗主栽品种ROC22中克隆出D14同源基因的cDNA全长,命名为ScHTD2,并对其进行生物信息学分析.结果表明：ScHTD2基因的cDNA全长为1 302 bp（KP137674.1）,具有927 bp的开放阅读框,编码308个氨基酸；蛋白质分析表明,ScHTD2为不稳定的水溶性非分泌蛋白,主要作用于氨基酸的生物合成或者作为生长因子调控生物生长发育.亚细胞定位于叶绿体,存在14个磷酸化位点,不存在乙酰化位点和信号肽.二级结构以α螺旋和无规则卷曲为主,还有一些残基形成β-折叠和链延伸.保守结构域和 结构预测均表明该蛋白包含一个α保守水解酶家族,属“D14-Like”或者“DAD2-Like”蛋白家族.推测ScHTD2可能作为独脚金内酯调控甘蔗分蘖信号转导中一个具有催化特性的水解脂酶而起作用.进化树分析表明该蛋白和高粱、玉米等单子叶植物进化关系较近.
关键词 甘蔗；独脚金内酯；ScHTD2；克隆；生物信息学分析
中图分类号 S566.1 文献标识码 A
Abstract D14 is a kind of hydrolysis esterase who can repress rice tillering effectively, which may be a vital component in strigolactons signal transduction pathways. In order to recognize the function characteristic of D14 homolog of sugarcane in regulating tillering, the cDNA of this gene named ScHTD2 was cloned from sugarcane variety ROC22 by using RT-PCR and RACE technology. The structure and function of ScHTD2 were analyzed using bioinformatics technology. The sequence analysis showed that ScHTD2 had a length of 1 302 bp containing an 927 bp open reading frame which encoded 308 amino acid residues. The characteristic analysis of protein structure using the bioinformatics method revealed that ScHTD2 was an unstable soluble non-secreted protein, which mainly plays a role in amino acid biosynthesis or regulating plant growth and development directly as a growth factor. Its protein is located in chloroplast, and has no acetylation loci and signal peptide but contained 14 phosphorylation sites. The secondary structure of ScHTD2 protein mainly encodes α-helix and random coil and still has some residues formed β- turn and chain extension. A α/β hydrolysis fold family contains in senior protein structure which belongs to“D14-like”or“DAD2-like”protein family. Based on the above we presumed that ScHTD2 may play a roles in the strigolactones signal transduction pathways as a catalytic hydrolysis esterase in regulating sugarcane tillering. Phylogenetic analysis showed that it had a closer evolutionary relationship with monocotyledon such as sorghum and maize.
Key words Sugarcane； Strigolactones； ScHTD2； Cloning； Bioinformatics analysis
doi 10.3969/j.issn.1000-2561.2016.06.014
中国食糖生产主要依靠甘蔗和甜菜作为原料,其中甘蔗糖占据了整个食糖产量的90%以上.甘蔗品种以有效茎作为收获产品,有效茎粗大而多是高产的重要指标,而大茎高产的关键则是在其分蘖期控制好分蘖芽的萌发[1].分蘖芽的萌发,受多种因素控制,如环境、水肥、生长阶段,内部调控因子和遗传因素等.近年来新发现的一种植物激素——独脚金内脂（Strigolactones）,能够抑制植物分枝（蘖）,它产生于植物根部,由于其向上运动,通过调节生长素和细胞分裂素的产生和运输进而起到抑制作用[2-3].
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结论：甘蔗ScHTD2基因克隆生物信息学分析为关于基因克隆方面的的相关大学硕士和相关本科毕业论文以及相关基因克隆技术的原理论文开题报告范文和职称论文写作参考文献资料下载。
