原创《不同真菌菌剂对烟草病毒病田间防治效果》：此文是一篇烟草病毒病论文范文,为你的毕业论文写作提供有价值的参考。
摘 要：真菌激发子能诱导植物产生系统抗性,表现出抗植物病毒的作用.对西瓜枯萎菌、苹果轮纹菌、小麦赤霉,以及土壤真菌E*-6菌株、XFPF-6菌株、C1菌株、XF*-8菌株、疫霉等8种真菌菌剂的抗病毒活性进行了研究.用不同真菌发酵液喷雾处理田间烟草植株叶片,检测真菌菌剂对烟草病毒病的防治效果.结果表明,8种真菌菌剂对烟草病毒病均有一定的防治效果,其中E*-6菌剂防效最高,发病高峰期的防效为83.8%.在烟草整个生长期真菌XFPF-6的防效较为稳定,防效稳定在60%以上.小麦赤霉菌,苹果轮纹菌的防效也较高,分别为64.8%,73.7%.除C1菌剂外,其他7种真菌菌剂的防效均高于盐酸吗啉胍,可开发为真菌抗病毒菌剂.经18s rRNA序列测定和显微形态观察鉴定E*-6为曲霉（Aspergillus sp.）,XF*-8为青霉（Penicillium sp.）.
关键词：真菌菌剂；烟草病毒病；防治效果
中图分类号：S435.72 文章编号：1007-5119（2015）04-0096-06 DOI：10.13496/j.issn.1007-5119.2015.04.017
Study on the Control Efficacy of Different Fungal Agents against Tobacco Viral Diseases
REN Jiaqing1, XUE Shoucong1, LI Xihong2, WEI Xiaohui3, HUANG Kai3, LIU Daisong3, SUN Liguang1,
ZHAO Xiuyun1*, XU Rubing2*
（1. College of Life Science and Technology, Huazhong Agricultural University,Wuhan 430070, China； 2.Tobacco Research Institute of Hubei Province, Wuhan 430030, China； 3. Shiyan Tobacco Company of Hubei, Shiyan, Hubei 442012, China）
Abstract： Fungal elicitors could induce plant systemic resistance and resistance against plant virus. The antiviral activities of Fusarium oxysporum, Physalospora piricola, Fusarium graminearum, and soil fungus strains E*-6, XFPF-6, C1, XF*-8 and Phytophthora sp. were studied. Fungi were cultured by liquid fermentation, the fermentation broth were collected and sprayed on leaves of tobacco plants. The antiviral effects of the fungi were determined via field experiments. The results showed that all eight fungal agents could control tobacco virus diseases. The controlling efficiency of strain E*-6 was the highest, 83.8% at the peak of disease incidence. The controlling efficiency of XFPF-6 was 60%, and was stable during the whole tobacco growth period. The controlling efficiency of F. graminearum and P. piricola were also high, being 64.8% and 73.7%, respectively. Except for strain C1, control efficiency of the other seven fungal agents were all higher than that of moroxydine hyrochloride. These fungi can be used in developing antiviral fungal agents. Based on 18s rRNA sequences of fungi and morphology observation, strain E*-6 was identified as Aspergillus sp., and strain XF*-8 identified as Penicillium sp.
Keywords： fungal agents； tobacco virus disease； controlling efficiency
烟草普通花叶病毒（Tobacco mosaic virus, TMV）、黄瓜花叶病毒（Cucumber mosaic virus, CMV）、马铃薯Y病毒（Potato virus Y,PVY）等3种病毒是引起我国烟草花叶病的主要病毒.TMV可侵染36科350多种植物,以烟草和番茄尤重[1].病毒病在我国烟田的发病率一般为5%～20%,重者达40%～50%,少数严重烟田在90%以上[2].发病后期,烟株严重矮化,生长缓慢.
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