原创《液相色谱串联质谱法测定猪肉中克伦特罗手性对映体残留量》：本论文主要论述了对映体论文范文相关的参考文献,对您的论文写作有参考作用。
摘 要：建立测定猪肉中克伦特罗手性对映异构体残留量的液相色谱串联质谱分析方法.5 g猪肉样品在碱化的条件下用乙酸乙酯提取,提取后用稀盐酸进行反萃取,萃取液过SCX固相萃取小柱,最后用5%氨化甲醇洗脱,洗脱液经氮气吹干后用500 μL甲醇（含0.02%的乙酸和0.1%的乙酸铵）定容.采用Astec CHIROBIOTICTM V手性色谱柱,以甲醇∶冰乙酸∶乙酸铵等于99.88∶0.02∶0.10（V/V）为流动相进行液相分离,电喷雾正离子（ESI+）模式电离,多反应监测（MRM）模式检测,内标法定量.克伦特罗单一对映体在2.0～200 μg/L质量浓度范围内呈良好的线性,线性相关系数均大于0.997 0,猪肉样品中克伦特罗最低检测限和定量限分别为0.10 μg/kg和0.25 μg/kg.猪肉中克伦特罗手性对映体在0.25～0.75 μg/kg范围内的回收率为95.8%～102.1%之间,相对标准偏差均小于5%.
关键词：克伦特罗；手性对映体；液相色谱串联质谱法；固相萃取
Determination of Residual Clenbuterol Enantiomers in Pork by Liquid Chromatography with Tandem Mass Spectrometry
CHEN Guo1, LIU Yongjun2, Lü Yan1, SUN Yami1, WU Yinliang1,*
（1. The Ningbo Academy of Agricultural Sciences, Ningbo 315040, China；
2. The Center for Animal Disease Control and Prevention of China, Beijing 100125, China ）
Abstract： A method was developed for determining residual clenbuterol enantiomers in pork by liquid chromatography with tandem mass spectrometry. Five grams of samples were extracted with ethyl acetate under basic condition. Then, clenbuterol enantiomers were back-extracted from the extracts using diluted hydrochloric acid. The hydrochloric acid extract was purified by SCX solid phase extraction （SPE） cartridge. The eluent was dried by blowing nitrogen and the residue was dissolved in 500 μL of methanol with 0.02% acetic acid and 0.1% ammonium acetate. The analytes were analyzed by LC-MS/MS on a chiral column （Astec CHIOBIOTICTM V） with a mixture of methanol-acetic acid-ammonium acetate as the mobile phase and quantified by the internal standard calibration curve method. Good linearities were obtained for two enantiomers in the concentration range of 2.0 – 200 μg/L with a correlation coefficient more than 0.997 0. The recoveries for the two enantiomers in pork were 95.8% – 102.1% at the fortified levels of 0.25 – 0.75 μg/kg with relative standard deviations less than 5%. The limits of detection （LOD） and quantitation （LOQ）were 0.10 and 0.25 μg/kg, respectively.
Key words： clenbuterol； enantiomers； liquid chromatography with tandem mass spectrometry； solid phase extraction
中图分类号：TS251.7 文献标志码：A文章编号：1001-8123（2015）05-0022-05
doi： 10.7506/rlyj1001-8123-201505006
克伦特罗（clenbuterol）俗称“瘦肉精”,曾常被非法应用于畜牧业,用来提高酮体瘦肉率.然而,由于克伦特罗易在动物组织中形成残留,曾多次发生严重的人畜中毒事故.目前,我国和欧盟已禁止克伦特罗作为生长促进剂使用.然而在评估克伦特罗对人体健康造成的危害时,往往对该药物作为单一化合物来看待,未考虑其是手性药物这一特征.而手性药物不同异构体之间在毒性、致畸、致癌、致突变上往往存在差异,必然导致对其危害估计不准确.因此若要更加科学评估该药物对人体健康造成的危害,首先应建立该药物在动物组织中的对映体残留分析方法.
对映体论文参考资料：
结论：液相色谱串联质谱法测定猪肉中克伦特罗手性对映体残留量为适合对映体论文写作的大学硕士及相关本科毕业论文，相关内消旋体开题报告范文和学术职称论文参考文献下载。
