原创《烟草赤星病菌嘧菌酯抗性突变体诱导其生物学特性》：本论文可用于嘧菌酯论文范文参考下载，嘧菌酯相关论文写作参考研究。
摘 要：为探究烟草赤星病菌对嘧菌酯的抗药性风险水平,通过对烟草赤星病菌在室内含药平板上的菌丝药剂驯服和分生孢子紫外诱变,进行突变体的生物学特性的研究,并对突变体靶标基因细胞色素b进行了测序分析.获得了4株嘧菌酯抗药性突变体.其中,菌丝药剂驯服未获得突变体；孢子紫外诱变获得4株突变体,突变率约为0.004%,突变体抗性倍数分别为42.50、282.50、515和107.50.4株突变体均降低了对嘧菌酯的敏感性.适合度研究表明,抗药性突变体具有同敏感菌株同样的分生孢子萌发能力和致病能力；菌丝生长速率较敏感菌株有所降低,3株突变体的分生孢子产量较敏感菌株增强,1株突变体较敏感菌株减弱.抗药性靶基因细胞色素b基因部分序列分析表明,突变体在129位和143位的碱基和敏感菌株完全相同,未发生任何突变.初步认为烟草赤星病菌对嘧菌酯的抗性风险水平较低.
关键词：嘧菌酯；抗药性；烟草赤星病菌；甲氧基丙烯酸酯；突变体
中图分类号：S435.72 文章编号：1007-5119（2016）06-0066-06 DOI：10.13496/j.issn.1007-5119.2016.06.012
Induction and Characterization of Laboratory Mutants of Alternaria alternata Resistant to Azoxystrobin
WANG Hancheng1, XUE Juanjuan2, HUANG Yanfei2, WANG Maosheng1, ZHANG Changqing2
（1. Guizhou Academy of Tobacco Science, Guiyang 550081, China； 2. College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China）
Abstract： This study was conducted to analyze the risk of resistance of Alternaria alternata to azoxystrobin. Resistant mutants were induced by incubating mycelial plugs and mutating conidia on poisoned agar plates. Mutant characterization and mechanism of resistance were also studied and the target gene cyt b was analyzed using partial sequence analysis. Four mutants of A. alternata with resistance to azoxystrobin were isolated. No mutant was obtained through mycelial plug incubation while four mutants were isolated through conidia mutation, with a frequency of around 0.004%. The resistance factors for the four mutants were 42.50, 282.50, 515 and 107.50, respectively. All resistant strains displayed a highly reduced sensitivity to azoxystrobin. Studies of fitness parameters in the wild-type and mutant strains of A. alternata showed that all resistant isolates had equal abilities on conidia germination and virulence, and less ability in mycelial growth. Three mutants showed enhanced ability of conidia production, while the other one decreased its’ conidia production. Sequence analysis of the target gene cyt b showed that there was no point mutation at position 129 or 143 on neither the four mutants or the sensitive isolate of A. alternata. This is believed to be the first report of different levels of azoxystrobin resistance in A. alternata. These studies suggested that the risk of resistance development in A. alternata was low for azoxystrobin.
Keywords： azoxystrobin； fungicide resistance； Alternaria alternata； strobilurin fungicide； mutant
烤烟（Nicotiana tabacum L.）是我国重要的经济作物,其在我国的产量和消费量均占全球约40% 左右[1-2].由Alternaria alternata（Frises）Keissler引起的烟草赤星病是烟草生长中后期发生最为严重的叶部病害之一,导致烟叶品质下降,烘烤价值降低[3-5].该病害具有间歇性和爆发性的特点,严重时烟叶发病率可高达90%,重病区减产、减收达50%以上[6-7].长期以来,烟草赤星病严重威胁我国烟草的生产.目前,我国主要采用菌核净（dimetachlone）来防治烟草赤星病,长期高频率的应用已使病原菌
嘧菌酯论文参考资料：
结论：烟草赤星病菌嘧菌酯抗性突变体诱导其生物学特性为关于本文可作为嘧菌酯方面的大学硕士与本科毕业论文嘧菌酯不能和什么药配论文开题报告范文和职称论文论文写作参考文献下载。
