原创《苗药痛风停对SD大鼠急性痛风性关节炎影响》：关于免费痛风性关节炎论文范文在这里免费下载与阅读,为您的痛风性关节炎相关论文写作提供资料。
【摘 要】目的：观察苗药痛风停对尿酸盐结晶诱导的SD模型大鼠急性痛风性关节炎的关节肿胀度、步态分级、滑膜病理、白细胞介素-1β（IL-1β）、IL-6及肿瘤坏死因子-α（TNF-α）的影响,探讨苗药痛风停对急性痛风性关节炎的作用机制.方法：将60只SD大鼠随机分为空白对照组,苗药痛风停低、中、高剂量组,双氯芬酸钠组,模型对照组,每组10只.除空白对照组外,其余分别建立以尿酸盐结晶诱导的大鼠急性痛风性关节炎模型,分别观察和测量造模前及造模后6,12,24,48 h大鼠关节横径,并计算关节肿胀度；观察大鼠24,48 h步态分级；造模后48 h处死大鼠,用酶联免疫吸附法测定细胞因子IL-1β、IL-6和TNF-α的表达.结果：各治疗组对大鼠关节肿胀度、步态分级、滑膜病理改变和IL-1β、IL-6、TNF-α的表达有不同程度的改善,苗药痛风停中、高剂量组和双氯芬酸钠组比较,差异无统计学意义（P > 0.05）.结论：苗药痛风停能够改善尿酸盐结晶诱导的SD模型大鼠急性痛风性关节炎关节肿胀度、步态分级及滑膜病理改变；苗药痛风停降低尿酸盐结晶诱导的SD模型大鼠细胞因子IL-1β、IL-6及TNF-α的表达,以中、高剂量作用明显,并呈剂量依赖性.
【关键词】 痛风性关节炎；细胞因子；苗药痛风停；尿酸盐；实验研究
doi：10.3969/j.issn.2095-4174.2015.08.002
【ABSTRACT】 Objective：To observe the effect of Miao medicine Tongfengting （苗药痛风停） on joint swelling,gait grading,pathological change of synovium,and the expression of IL-1β,IL-6 and TNF-α of SD rats with acute gout arthritis induced by monosodium urate crystal in order to study the mechanism of action of the medicine on acute gout arthritis.Methods：60 SD rats were randomly divided into a blank control group,low,medium and high dose groups of Miao medicine Tongfengting,a diclofenac sodium group and a model control group,10 rats in each group.Apart from the control group,models of acute gout arthritis induced bymonosodium urate crystal were established.The joint transverse diameter of model rats was observed and the joint swelling was measured respectively after 6,12,24 and 48 hours.The model rats were killed after 48 hours to measure the expression of IL-1β,IL-6 and TNF-α.Results：The joint swelling,gait grading,pathological change of synovium,IL-1β,IL-6 and TNF-α of SD rats all had different degrees of improvement in each treatment group.The difference between the medium and high dose groups and the diclofenac sodium group was not statistically significant （P > 0.05）.Conclusion：Miao medicine Tongfengting can improve the joint swelling,gait grading and pathological change of synovium and lower the expression of IL-1β,IL-6 and TNF-α which was obvious and dose dependent in the medium and high dose groups.
【Keywords】gout；cytokine；Miao medicine Tongfengting（苗药痛风停）；monosodium urate；experimental study
痛风（gout）是体内嘌呤代谢紊乱及/或排泄减少导致尿酸盐（monosodium urate,MSU）沉积所引起的晶体性关节病.痛风在我国的患病率约为0.15%～0.67%,较以前有明显升高[1].痛风性关节炎常常合并有心血管疾病、高血压、2型糖尿病、肥胖、高脂血症、代谢综合征、慢性肾脏疾病和肾结石等[2],而这些合并症往往是NSAIDs和/或秋水仙碱常见的禁忌症[3].细胞因子白细胞介素IL-1β、IL-6、IL-8、TNF-α等的高表达可导致、加重MSU结晶诱导的急性炎症的发生和发展[4].中医学对痛风治疗有深入的认识,积累了丰富的临床经验.苗药痛风停根据中医理论和苗医理论对痛风认识的结合而遣方组药,由白虎加桂枝汤加减苗药组成,通过本课题组多年临床观察、临床研究和实验研究,其治疗急性痛风性关节炎疗效显著,安全性好,缓解痛风性关节炎效果明显,并具有降尿酸作用[5-7].在前期实验研究中,笔者已经证实苗药痛风停能够抑制SD大鼠急性痛风性关节炎IL-8、环氧化酶-2（COX-2）的表达,减轻关节滑膜炎症[8-9].本文旨在探讨苗药痛风停对IL-1β、IL-6和TNF-α的影响.
痛风性关节炎论文参考资料：
结论：苗药痛风停对SD大鼠急性痛风性关节炎影响为关于本文可作为相关专业痛风性关节炎论文写作研究的大学硕士与本科毕业论文痛风性关节炎外用药论文开题报告范文和职称论文参考文献资料。
