原创《禽肉中铜绿假单胞菌分离其耐药性》：本论文可用于禽肉论文范文参考下载，禽肉相关论文写作参考研究。
摘 要：采用GB 4789.4—2010《食品安全国家标准 食品微生物学检验 沙门氏菌检验》中的选择性前增菌方法从鲜鹅肉、鸭肉样本中检测到2 株疑似沙门氏菌的菌株,但经ATB生化鉴定和基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization-time of flight-mass spectrometry,MALDI-TOF-MS）鉴定为铜绿假单胞菌.比对2 株铜绿假单胞菌和沙门氏菌的生化图谱,其中鸟氨酸脱羧酶、精氨酸双水解酶和L-阿拉伯糖的实验结果同为阳性,其余生化反应结果差异较大；2 株铜绿假单胞菌的质谱标识峰足迹与肠炎沙门氏菌CICC21482有明显不同.微生物耐药实验结果表明：大部分抗生素对铜绿假单胞菌无抑制作用,只有喹诺酮类抗生素环丙沙星、氨基糖苷类抗生素庆大霉素和链霉素对其有一定的抑制作用.
关键词：禽肉；铜绿假单胞菌；沙门氏菌；生化鉴定；基质辅助激光解吸电离飞行时间质谱
Isolation and Drug Resistance of Pseudomonas aeruginosa from Poultry Meat
WEI Chao1,2, DAI Xiaohang1,2, GUO Lingan1,2, LEI Xinyu1, LIU Yueyue1,*
（1.Analysis and Testing Center of Sichuan Academy of Agricultural Sciences, Chengdu 610066, China；
2.Agricultural Products Quality and Safety Risk Assessment Laboratory of Ministry of Agriculture （Chengdu）, Chengdu 610066, China）
Abstract： Two strains of suspected Salmonella spp were detected from fresh goose and duck samples by the selective
pre-enrichment method according to the GB 4789.4?2010. However, the microorganism was identified as Pseudomonas aeruginosa by ATB biochemical identification and matrix-assisted laser desorption-ionization time of flight mass spectrometry （MALDI-TOF-MS）. By analyzing the biochemical profiles of the two Pseudomonas aeruginosa strains and Salmonella spp, we found that the results of ornithine decarboxylase, arginine dihydrolase and L-arabinose tests were identically positive while the other biochemical results varied widely. Besides, the footprints of mass spectrometric peak of Pseudomonas aeruginosa and Salmonella enteritidis were significantly different. Drug resistance results showed that most of the antibiotics examined except ciprofloxacin, gentamicin and streptomycin could not inhibit Pseudomonas aeruginosa.
Key words： poultry meat； Pseudomonas aeruginosa； Salmonella； biochemical identification； matrix-assisted laser desorption-ionization time of flight mass spectrometry （MALDI-TOF-MS）
DOI：10.7506/rlyj1001-8123-201707002
中图分类号：TS251.1 文献标志码：A文章编号：1001-8123（2017）07-0007-04
引文格式：
魏超, 代晓航, 郭灵安, 等. 禽肉中铜绿假单胞菌的分离及其耐药性[J]. 肉类研究, 2017, 31（7）： 7-10. DOI：10.7506/rlyj1001-8123-201707002. http：//www.rlyj.pub
WEI Chao, DAI Xiaohang, GUO Lingan, et al. Isolation and drug resistance of Pseudomonas aeruginosa from poultry meat[J]. Meat Research, 2017, 31（7）： 7-10. DOI：10.7506/rlyj1001-8123-201707002. http：//www.rlyj.pub
銅绿假单胞菌（Pseudomonas aeruginosa）是革兰氏阴性菌,专性需氧,菌体长短不一,成对或呈短链状排列,单鞭毛、无芽胞、无荚膜,在普通培养基上可以产生水溶性色素,如绿脓素（pyocynin）等,是一种常见的条件致病菌[1].铜绿假单胞菌的生长温度范围为25～42 ℃,该菌具有4 ℃不生长而在42 ℃可以生长的特点[2].铜绿假单胞菌是医院的主要污染菌,感染可发生于很多解剖部位,包括皮肤、骨、耳、眼、尿路和心脏瓣膜等,感染部位与细菌的感染程度及病人的易感性
禽肉论文参考资料：
结论：禽肉中铜绿假单胞菌分离其耐药性为关于本文可作为禽肉方面的大学硕士与本科毕业论文禽肉的价格论文开题报告范文和职称论文论文写作参考文献下载。
