原创《透骨消痛胶囊对膝骨关节炎大鼠内质网应激影响》：本论文为您写内质网毕业论文范文和职称论文提供相关论文参考文献，可免费下载。
【摘 要】目的：观察透骨消痛胶囊对膝骨关节炎大鼠软骨内质网应激的影响,探讨透骨消痛胶囊治疗
骨关节炎的可能机制.方法：将30只清洁级SD大鼠随机分为正常对照组,模型对照组,透骨消痛胶囊高、中、低剂量组,每组6只.除正常对照组外,其余各组采用质量分数为4%的木瓜蛋白酶注射建立大鼠膝骨关节炎模型.给药8周后,收集大鼠右侧股骨髁和胫骨平台,光镜检查软骨组织的形态学变化,利用原位末端标记法（TdT-mediated dUTP nick-end labeling,TUNEL）检测软骨细胞凋亡情况,免疫组化检测软骨组织内质网应激蛋白激酶（PKR-like ER kinase,PERK）、真核启动因子2α（eukaryotic translation initiation factor 2α,eIF2α）、转录活化因子4（activating transcription factor 4,ATF4）、免疫球蛋白结合蛋白（immunoglobulin binding protein,Bip）、生长抑制DNA损伤基因153（growth arrest and DNA-damage-inducible gene 153,
GADD153）、软骨细胞功能基因Ⅱ型胶原蛋白（CollageⅡ）和转录因子SOX-9蛋白的表达.结果：透骨消痛胶囊对大鼠骨关节炎模型关节软骨的大体外观及HE染色均有明显改善,TUNEL结果显示其能够降低软骨细胞凋亡指数（P < 0.01）,免疫组化结果显示透骨消痛胶囊较模型对照组能够下调PERK、eIF2α、ATF4、Bip及GADD153蛋白表达,上调软骨细胞功能基因CollageⅡ和SOX-9的蛋白表达
（P < 0.05或P < 0.01）.结论：透骨消痛胶囊可通过抑制内质网应激的信号转导,减少软骨细胞的凋亡数目,改善软骨细胞形态,促进软骨细胞代谢及软骨修复,以维持软骨结构的相对完整,从而延缓关节软骨退变.
【关键词】 骨关节炎,膝；透骨消痛胶囊；内质网应激；信号转导；大鼠
doi：10.3969/j.issn.2095-4174.2015.11.001
Study on the Effect of Tougu Xiaotong Capsule（透骨消痛胶囊）on CartilageEndoplasmic Reticulum
Stress in Rats with Knee Osteoarthritis
YE Jin-xia,WU Guang-wen,LAI Shun-miao,ZHENG Chun-song,LI Xi-hai,LIU Xian-xiang,YE Hong-zhi
【ABSTRACT】 Objective：To observe the effect of Tougu Xiaotong Capsule （透骨消痛胶囊,TXC） on cartilage endoplasmic reticulum stress in rats with knee osteoarthritis in order to study its possible mechanism of treating osteoarthritis.Methods：Thirty clean SD rats were randomly divided into a normal control group,a model control group,and high,medium and low dose groups of TXC （HTXC group,MTXC group and LTXC group）,
6 rats in each group.Except for the normal control group,the rest groups were given injections of 4% papain to establish rat models of knee osteoarthritis.After 8 weeks of medication,right lateral thighs and tibial plateaus of rats were collected to detect the morphological changes of cartilage tissue with light microscope.TUNEL （TdT-mediated UTP nick-end labeling） was used to detect chondrocyte apoptosis.Immunohistochemisty was used to detect the expressions of PERK （PKR-like ER kinase）,eIF2α （eukaryotic translation initiation factor 2α）,ATF4 （activating transcription factor 4）,Bip （immunoglobulin binding protein）,GADD153 （growth arrest and DNA-damage-inducible gene 153）,CollageⅡ and transcription factor SOX-9.Results：TXC could significantly improve the general appearance and HE staining of articular cartilage of rat models.The results of TUNEL showed the decrease of chondrocyte apoptosis index （P < 0.01）.Immunohistochemistry results showed the down-regulation of the expression of PERK,eIF2α,ATF4,Bip and GADD153 and the up-regulation of the expression of CollageⅡ and SOX-9 （P < 0.05 or P < 0.01）.Conclusion：TXC,by way of inhibiting endoplasmic reticulum stress signal transduction,can reduce the number of chondrocyte apoptosis,improve the morphology of chondrocytes,promote the metabolism of chondrocytes and cartilage repair,and maintain relatively intact cartilage structure,to delay the degeneration of articular cartilage.
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结论：透骨消痛胶囊对膝骨关节炎大鼠内质网应激影响为适合不知如何写内质网方面的相关专业大学硕士和本科毕业论文以及关于高尔基体论文开题报告范文和相关职称论文写作参考文献资料下载。
