原创《高效液相色谱法测定土壤中2，4—二羟基苯甲酸含量》：本论文可用于色谱法论文范文参考下载，色谱法相关论文写作参考研究。
摘 要：
建立高效液相色谱法（HPLC）测定土壤中2,4-二羟基苯甲酸含量,分析4种不同土地利用方式土壤中2,4-二羟基苯甲酸的垂直分布趋势.通过用乙酸乙酯水浴24 h提取2,4-二羟基苯甲酸,蒸干并溶解于色谱级甲醇中.以流动相为3%冰醋酸和甲醇（75：25）对样品进行洗脱,流速1 mL/min,色谱柱为Agilent ZORBAX SB-C18（4.6 mm×150 mm,5μm）,检测波长280 nm,柱温25℃.结果表明：2,4-二羟基苯甲酸进样浓度在80～800μg/mL范围内和峰面积呈良好的线性关系,线性回归方程为Y等于13.315X-12.803（r2等于0.999 9）；2,4-二羟基苯甲酸的重复性、精密度和稳定性,相对标准偏差（RSD）结果分别为0.778%、1.623%和1.918%,平均加样回收率为97.675%,RSD结果为0.771%（n等于6）,均达到定性和定量分析的要求.应用高效液相色谱法测定2,4-二羟基苯甲酸含量操作简便、快速、准确,天然次生林土壤中的2,4-二羟基苯甲酸含量大于其余3种土地利用方式的,且4种土地利用方式皆表现出随着土层深度的增加,2,4-二羟基苯甲酸含量降低的趋势.
关键词：
高效液相色谱法；2,4-二羟基苯甲酸；土地利用方式
中图分类号：TS 47；O 657.72 文献标识码：A 文章编号：1001-005X（2016）06-0034-04
Determination of 2,4-dihydroxy Benzoic AcidConcentration of Soil Using HPLC Method
He Chunting1,Zhang Yumei1,Ji Li2,Yang Lixue2*
（1.Nujiang Lisu Autonomous Prefecture,Yunnan province,Forestry Bureau,Nujiang 673100；
2.Northeast Forestry University,College of Forestry,Harbin 150040）
Abstract：
The concentration of 2,4-dihydroxy benzoic acid was determined based on the established High Performance Liquid Chromatography （HPLC） method and the amount of 2,4-dihydroxy benzoic acid was analyzed in 4 different land uses in the vertical distribution.The 2,4-dihydroxy benzoic acid in sample was extracted by 24 hours-water-bath heating with ethyl acetate,and then was evaporated and dissolved in chromatographic grade methanol.The sample elution was taken by the mobile phase which was composed of 3% glacial acetic acid and methanol （75∶25） with the flowing rate of 1 mL/min.The chromatographic column was Agilent ZORBAX SB-C18（4.6 mm×150 mm,5 μm） while the detecting wavelength was 280 nm and the column temperature was 25℃.The result showed that a good linear relationship existed between the concentration of 2,4-dihydroxy benzoic acid and the peak area in the range of 80～800 μg/mL.The obtained linear regression equation was Y等于13.315X-12.803 （r2等于0.999 9）.The relative standard deviation （RSD） of repeatability,precision and stability of 2,4-dihydroxy benzoic acid were 0.778%,1.623% and 1.918% respectively.The average recovery rate was 97.675% and the RSD was 0.771% （n等于6）,all of which met the requirements of qualitative and quantitative analysis.The determination of 2,4-dihydroxy benzoic acid content using HPLC method was simple,rapid and accurate.The amount of the 2,4-dihydroxy benzoic acid in soil of natural secondary forest was found the greatest among 4 kinds of land.A uses and the concentration of 2,4-dihydroxy benzoic acid decreased with the depth of the soil.
色谱法论文参考资料：
结论：高效液相色谱法测定土壤中2，4—二羟基苯甲酸含量为关于色谱法方面的论文题目、论文提纲、色谱法的基本原理图解论文开题报告、文献综述、参考文献的相关大学硕士和本科毕业论文。
